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KMID : 0375319960180020393
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Journal of Clinical Pathology and Quality Control
1996 Volume.18 No. 2 p.393 ~ p.400
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Urinary Protein Quantitation with a Pyrogallol Red-Molybdate Complex, Manually and in a Hitachl 747 Chemistry Autoanalyser
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신정원
박남재/이정운/임환섭/박영옥/권오헌
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Abstract
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Background:
As the number of urinary protein quantitation are increasing in demand that we attempt to adopt method using chemistry autoanalyser. We compared tubidimetric method (TCA) with automated analyser method using pyrogallol red-molybdate complex
(PRM)
reagent.
Methods:
PRM reagent was prepared by mixing 1.5mmlo/L of pyreogallol red solution with 10mmol/L of molybdate solution. The calibration curve was established with bovine serum albumin solutions with concentrations ranging from 0.0625 to 2.0g/L. This
range of
standards were selected for evaluation of linearity and precision. Also, recovery rate of albumin and globulin were determined. Random urine samples from 110 normal con trols were used for estimation of reference range in urinary protein. From
126
admission patients, timed collection of 24 hour urine samples were used for comparison between TCV and automated PRM methods.
Results:
From standard albumin solution, 10 replicates evaluated in each concentration of 0.5g/L, 1.0g/L that showed total precision of 6.87% and 4.1%, respectively. regression of standard aalbumin solution showed y=0.2744×-0.0099 with correlation
coefficient of 0.9999. Recovery rate for albumin solution ranged from 91.37% to 96.22% and globulin was 77.12%. results of comparison study between TCA and automated method showed y=0.7321×+0.1672 with correlation coefficient of 0.9904.
reference
range
of urinary protein for normal control was 17.1~185.7mg protein/g creatinine
Conclusions:
since PRM reagent does not adsorb onto the wall of reaction cuvette, this method showed wide analytical range with good sensitivity and specificity. As our study revealed good correlation between TCA and automated method that automated
urinary
protein quantitation could be adopted to chemistry autoanalyser. Large number of urinary protein could be quantitated easily using dye-binding method in chemistry autoanalyser.
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